Stephan Tirier

Research Interest

  • Analysis of tumor cell heterogeneity in solid tumors and hematopoietic malignancies
  • Single cell (epi)genomics and its applications in translational/clinical research
  • Methods for spatially resolved analysis of gene expression
  • Single cell analysis at the interface between genomics and imaging

Scientific Background

  • since 09/2018: Postdoc at the German Cancer Research center (Heidelberg, Germany) in the division of Chromatin Networks of Prof. Dr. Karsten Rippe
  • 04/2015 - 08/2018: PhD student at the German Cancer Research center (Heidelberg, Germany) in the division of Theoretical Bioinformatics of Prof. Dr. Roland Eils in the intelligent imaging group of Dr. Christian Conrad
  • 08/2012 - 12/2012: Erasmus internship in the laboratory of Prof. Dr. Birgitta Henriques-Normark and Prof. Dr. Staffan Normark (Microbial Pathogens); Karolinska Institute, Department of Microbiology, Tumor and Cell Biology, Stockholm, Sweden
  • 10/2011 - 06/2014: University of Wuerzburg - Master program Biology (Major: Microbiology and Molecular Cell- and Developmental Biology; Thesis in the Young Investigator Group of Dr. Cynthia Sharma, Research Center for Infectious Diseases (ZINF) at the University of Wuerzburg, Germany
  • 10/2008 - 07/2011: University of Wuerzburg - Bachelor program Biology (Major: Microbiology; Thesis in the Department of Microbiology of Prof. Dr. Thomas Rudel at the University of Wuerzburg, Germany


  • Tirier, S.M. ... Rippe, K., Raab, M.S. (2019) Dissecting Heterogeneity of Tumor Cells and Their Microenvironment in Refractory Multiple Myeloma. Blood, 134(Supplement_1), 571.
  • Tirier, S.M., ... Rippe, K., ...Conrad, C. (2019) Pheno-seq - linking visual features and gene expression in 3D cell culture systems. Scientific Reports, 9(1), 1-15.
  • Krieger, T. G.,Tirier, S.M., ... Conrad, C. (2019). Modeling glioblastoma invasion using human brain organoids and single-cell transcriptomics. BioRxiv, 630202.
  • Waschow, M., Wang, Q., Saary, P., Klein, C., Aschenbrenner, S., Jechow, K., Meier, L., Tirier, S.M. ..., Conrad, C. (2019). Induced aneuploidy disrupts MCF10A acini formation and CCND1 expression. BioRxiv, 2019.12.15.876763.
  • Pernitzsch, S. R.,Tirier, S.M., Beier, D. & Sharma, C. M. A variable homopolymeric G-repeat defines small RNA-mediated posttranscriptional regulation of a chemotaxis receptor in Helicobacter pylori. Proc. Natl. Acad. Sci. 111, E501-E510 (2014).
  • Balaban, M., Baettig, P., Muschiol, S.,Tirier, S.M., Wartha, F., Normark, S., Henriques-Normark, B. Secretion of a pneumococcal type II secretion system pilus correlates with DNA uptake during transformation. Proc. Natl. Acad. Sci. 111, E758-E765 (2014).


Stephan Tirier

DKFZ & BioQuant Center

Division of Chromatin Networks

Im Neuenheimer Feld 267-BQ24

69120 Heidelberg


BioQuant room 641

Tel.: +49-6221-54-51377

Fax: +49 6221 54 51487

e-mail: s.tirier (at)