BIOQUANT > Research Groups > Genome Organization & Function > Teaching > Lab courses and practicals

Analytical ultracentrifugation

Program

Monday

13:00-15:00 Theory of analytical ultracentifugation
15:00-15:30 Break
15:30-16:00 Check-out the centrifuge
16:00-17:00 Experimental strategies and intro to biological system
17:00 - ... Design of experiments

Tuesday

9:00-12:00 Assembly of cells, start velocity run
12:00-14:00 Lunch Break
14:00-16:00 Software setup and tutorial
16:00-17:30 Start second run (optional)

Wednesday

10:00-12:00 Data analysis
12:00-14:00 Break
14:00-16:00 Presentation and discussion of results
16:00 cell disassembly

Group 1

Yu-Wei Shieh, Cornelia Klein
sample: a) 0.2 EDTA; b) + 1 mM MgCl2

Group 2

Christian Duellberg; Rajesh Bhardwaj
sample a) 50 mM NaCl; b) + glutaraldehyde for fixation

Group 3

Barbara Roider; Vladimir Teif
sample: a) fibers + H1 in 50 mM NaCl; b) add NaCl to 500 mM to induce dissociation of H1

Slides and handouts

Papers

Links

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