Methods

We apply and develop the experimental and theoretical methods listed below to analyze chromatin networks and genome functions that are described under research topics.

Quantitative microscopy

• Automated fluorescence imaging
• Fluorescence correlation spectroscopy
• Fluorescence bleaching techniques

Single Cell Sequencing

• single cell RNA-seq with 5'-UMI
• single cell RNA-seq of full length transcripts
• single cell ATAC-seq to map open chromatin loci

Chromatin editing

• Optogenetics in the nucleus
• Lac operator (lacO)/ Lac repressor (LacI) system
• dCas9 mediated editing
• Microinjection

Network modeling

• Chromatin network models
• Transcription factor activity computation
• Data analysis of single cell sequencing data

Software download

• FREDIS (FRAP Reaction Diffusion Solver)
• MCORE (Multi-scale CORrelation Evaluation)

Material request

Plasmid vectors from our publications are available on request or via Addgene at https://www.addgene.org/Karsten_Rippe/ .