BIOQUANT

Research Projects

As an interdisciplinary group that integrates molecular/cell biology, physics and chemistry we deal with diverse methods and technologies to investigate the complex organizational and structural details of chromatin. The following list covers the main projects we are currently working on. For an overview of techniques that are used within the group actively or passively please have a look at Methods.

Epigenetic Networks and Signal Transduction

- Analysis of epigenetic networks in living cells

- Aberrant signal transduction in chronic lympocytic leukaemia

- Chromatin features associated with the plasticity of the stem cell state

A comprehensive analysis of chromatin networks chronic lymphocytic leukemia is conducted within a BMBF consortium project coordinated by Karsten Rippe and Daniel Mertens. For more information see www.CancerEpiSys.org

Alternative Lengthening of Telomeres

In vivo recruitment and high-throughput screening of protein factors involved in the Alternative Lengthening of Telomeres (ALT) pathway in telomerase-negative cancer cells.

A comprehensive analysis of telomere maintenance mechanisms and telomeric chromatin networks in glioblastoma and protstate cancer is conducted within a BMBF consortium project. The project is coordinated by Karsten Rippe and is part of the e:Med Systems Medicine Program. For more information see www.CancerTelSys.org

Chromatin Remodelers

RNA in Chromatin Structure

In vivo investigation of the structural importance of RNAs for the maintenance of chromatin structure.

Multifocal Fluorescence Fluctuation Microscopy

Further development and application of our self-designed spatiotemporal fluctuation microscope (STFM). Investigation of new methods for mapping protein mobility and interactions in living cells.


Theoretical approaches, Simulation and Modeling

Investigation of chromatin folding and mechanical properties with Monte-Carlo Simulations of chromatin fibers; Molecular Dynamics Simulations of single nucleosomes and DNA-protein interactions as well as analysis of DNA Lattice Binding Models.