Transcriptional silencing through histone lysine methylation by chromatin-bound Suv39h complexes has mostly been studied in mouse pericentric heterochromatin (PCH), which is mainly composed of minor and major satellite repeats. PCH is an interesting model system for studying gene silencing as the heterochromatic state suppresses repeat transcription and PCH can be easily identified on fluorescence microscopy images.
Protein factors that set, maintain and propagate those marks were studied in our lab and we propose that a stably chromatin-bound complex of Suv39h is specific for PCH. It is postulated that Suv39h binding in this complex is stabilized by heterochromatin protein 1 (HP1) and methyl-binding proteins such as MBD1 and MeCP2, which specifically bind methylated DNA (5-methyl cytosine). In my PhD thesis work, I am characterizing this complex by combining advanced fluorescence microscopy-based methods such as fluorescence recovery after photobleaching and fluorescence (cross-)correlation spectroscopy for studies in living cells with deep sequencing.
A principal goal of my research is to study the role of Suv39h-mediated histone methylation outside of PCH. This involves a genome-wide colocalization study of the above-mentioned components by ChIP-seq. Thus, binding sites of the complex members outside of repetitive sequences will be detected. In addition, I am conducting a comparative transcriptome analysis by RNA-seq of wild-type and Suv39h double null mouse fibroblasts to identify transcripts that are silenced in a Suv39h-dependent manner. Thus, by combining chromatin interaction studies of PCH proteins with deep sequencing analyses, I will address whether the mechanism by which satellite transcripts are suppressed is also operational in other regions of the genome.
- since 03/2013: PhD student at the German Cancer Research center in the research group “Genome Organization & Function” (group leader Karsten Rippe)
- 08/2011 – 11/2011: Research intern in the “Hematopoiesis and Immunotherapy” group of Naomi Taylor at the Molecular Genetics Institute in Montpellier, France
- 03/2011 – 07/2011: Research intern in the “Soft matter” group of Gladys Massiera at the Charles Coulomb laboratory in Montpellier, France
- 03/2011 – 11/2011: University of Montpellier I/II (France) (European student exchange; ERASMUS)
- 10/2010 – 11/2012: University of Heidelberg - Master program of Molecular Biotechnology (major: biophysical chemistry; thesis in the “Biophysics of macromolecules group” of Jörg Langowski at the German Cancer Research center)
- 06/2009 – 10/2009: Member of the University of Heidelberg iGEM team 2009
- 05/2008 – 07/2009: Student research assistant (Cellzome AG – Heidelberg)
- 10/2007 – 08/2010: University of Heidelberg - Bachelor program of Molecular Biotechnology (major: biophysical chemistry; thesis in the „Microfluidics group“ of the “New materials and biosystems” department of Prof. Spatz at the Max Planck institute for intelligent systems and the university of Heidelberg)
- PhD scholarship of the Helmholtz International Graduate School for Cancer Research, 2013
- ERASMUS scholarship for European student exchange, 2011
- Exchange scholarship from the Studienstiftung des deutschen Volkes (German National Academic Foundation), 2011
- Scholarship from the University of Heidelberg for the tuition fees (awarded for outstanding course achievements), 2009
- Scholarship from the Studienstiftung des deutschen Volkes (German National Academic Foundation), 2008
- Müller-Ott, K., Erdel, F., Metveeva, A., Mallm, J.-P., Rademacher, A., Hahn, M., Bauer, C., Zhang, Q., Kaltofen, S., Schotta, G., Höfer, T. & Rippe, K. (2014) Specificity, propagation and memory of pericentric heterochromatin. Molecular Systems Biology 10(8): 746.
- Hofmann, T. W., Hänselmann, S., Janiesch, J.-W., Rademacher, A., & Böhm, C. H. J. (2012) Applying microdroplets as sensors for label-free detection of chemical reactions. Lab on a chip 12(5): 916–922.
DKFZ & BioQuant Center
Research Group Genome Organization & Function
Im Neuenheimer Feld 267-BQ24
BioQuant room 623
Fax: +49 6221 54 51487
e-mail: a.rademacher (at) dkfz.de